JoVE Journal

Immunology and Infection

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Nanyang Technological University

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Here, the protocol describes how to perform double labeling immunofluorescence using primary antibodies raised in the same species to study host-pathogen interactions. Also, it can include the third antibody from a different host in this protocol. This approach can be made in any cell type and pathogens.

Chapters in this video

0:04

Introduction

1:21

Parasite Cultures

2:00

Control Immunofluorescence Protocol

3:23

Double Labeling Immunofluorescence ProtocolUsing Monoclonal and Polyclonal Antibodies Raised in the Same Host

4:18

Triple Labeling Immunofluorescence Protocol with the Addition of the Third Polyclonal Antibody from Different Host Species

5:16

Confocal Imaging Acquisition

5:39

Results: Host-pathogen Interactions Studies Using Double Labeling Immunofluorescence

6:51

Conclusion

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