Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping
3.5K Views
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06:22 min
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October 27th, 2020
DOI :
October 27th, 2020
•Guadalupe Estrada-Gutierrez1, Eyerahi Bravo-Flores2, Veronica Ortega-Castillo3, Veronica Flores-Rueda4, Ismael Mancilla-Herrera5, Salvador Espino Y Sosa6, Maribel Sánchez-Martínez2, Otilia Perichart-Perera7, Mario Solis-Paredes8
1Research Division, Instituto Nacional de Perinatologia, 2Department of Immunobiochemistry, Instituto Nacional de Perinatologia, 3Department of Obstetrics, Instituto Nacional de Perinatologia, 4Department of Academic Programs and Continuing Education, Instituto Nacional de Perinatologia, 5Department of Infectology and Immunology, Instituto Nacional de Perinatologia, 6Clinical Research Branch, Instituto Nacional de Perinatologia, 7Department of Nutrition and Bioprogramming, Instituto Nacional de Perinatologia, 8Department of Human Genetics and Genomics, Instituto Nacional de Perinatologia, Mexico City, Mexico
Chapters in this video
0:00
Introduction
0:46
Visceral Adipose Tissue Collection and Enzymatic Digestion
2:10
RNA Extraction from Mature Adipocytes and microRNA Quantification Assay
3:16
Characterization of Macrophage Subsets from Stromal Vascular Fraction
4:42
Results: Identification of Macrophages from Visceral Adipose Tissue
6:02
Conclusion
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