Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana
7.3K Views
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13:29 min
•
January 16th, 2021
DOI :
January 16th, 2021
•Aigerim S. Kamzina*1,2,3, Michelle P. DiPalma*1,2,3, Joseph G. L. Hunter1,2,3, Andrew G. Diamos1,2,4, Boyd Armer2,3, Tsafrir S. Mor1,2,3, Hugh S. Mason1,2,3
1Center for Immunotherapy, Vaccines and Virotherapy, The Biodesign Institute, Arizona State University, 2School of Life Sciences, Arizona State University, 3Molecular Biosciences/Biotechnology Undergraduate Program, Arizona State University, 4Department of Microbiology and Immunology, University of Michigan Medical School
Chapters in this video
0:00
Introduction
0:38
Cultivate Nicotiana benthamiana Plants
1:53
Preparation of Agrobacterium tumefaciens for Infiltration
3:51
Transient Needle-less Agroinfiltration
5:24
Grow and Observe the Infiltrated N.benthamiana
6:10
Protein Extraction
7:56
Protein G Column Chromatography
11:23
Representative Results
12:56
Conclusion
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