JoVE Journal

Chemistry

This content is Open Access.

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

This protocol presents an approach to fingerprint and explore multi-dimensional data collected by comprehensive two-dimensional gas chromatography coupled to mass spectrometry. Dedicated pattern recognition algorithms (template matching) are applied to explore the chemical information encrypted in the extra-virgin olive oil volatile fraction (i.e., volatilome).

Chapters in this video

0:04

Introduction

1:45

Importing Raw Data

2:24

Baseline Correction

2:50

Chromatographic Image Coloring and 2D Peak Analyte Detection

3:49

2D Peak Filtration and Peak Spectra Location

4:54

Targeted Peak Template Creation and Template Matching and Application

6:20

Template Transformation (Optional)

6:58

Untargeted and Targeted Chromatogram Analysis

7:46

Results: Representative Chromatographic Fingerprinting

9:27

Conclusion

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