Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10.3K Views
•
10:52 min
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January 6th, 2016
DOI :
January 6th, 2016
•Chapters in this video
0:05
Title
0:58
Yeast Cell Culture and Radiolabeling of Phosphoinositides
2:58
Deacylation and Extraction of the Radiolabeled Phosphoinositides
5:14
Separation of Tritium-labeled Glycero-inositides by High Pressure Liquid Chromatography (HPLC)
7:15
Data Analysis
8:55
Results: HPLC Analysis of Radiolabeled Phosphoinositides
9:55
Conclusion
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