Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

6.1K Views

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07:36 min

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August 27th, 2020

DOI :

10.3791/61383-v

August 27th, 2020

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Katsunori Yanai¹, Shohei Kaneko¹, Hiroki Ishii¹, Akinori Aomatsu¹^,², Kiyonori Ito¹, Keiji Hirai¹, Susumu Ookawara¹, Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ²Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, ³Department of Medical Physiology, Meiji Pharmaceutical University

Summary

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Quantitative Real Time PCR

MicroRNA Expression

Mouse Kidney

Unilateral Ureteral Obstruction

Phenyl guanidine Lysis Reagent

Homogenization Process

Chapters in this video

0:04

Introduction

0:33

Sham and UUO Surgeries

1:52

Collection of Kidney Samples

2:39

Extraction of Total RNA from the Kidney Samples

5:28

Synthesis of cDNA

6:29

Results: Differentially Expressed miRNAs in the Kidneys of UUO Mice

7:08

Conclusion

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