Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining

10.5K Views

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10:40 min

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June 13th, 2020

DOI :

10.3791/61143-v

June 13th, 2020

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Teresa P. Silva¹^,², Tiago G. Fernandes¹, Diogo E. S. Nogueira¹, Carlos A. V. Rodrigues¹, Evguenia P. Bekman¹^,²^,³, Yas Hashimura⁴, Sunghoon Jung⁴, Brian Lee⁴, Maria Carmo-Fonseca², Joaquim M. S. Cabral¹

¹iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, ²Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, ³The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa, ⁴PBS Biotech, Inc, Camarillo, CA, USA

Summary

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Cerebellar Organoids

Human Pluripotent Stem Cells

IPSC

Scalable Generation

Bioreactor

Cell Detachment Medium

Differentiation Medium

RPM Agitation

Sucrose Treatment

Chapters in this video

0:04

Introduction

0:23

Seeding the Bioreactor

2:06

Differentiation and Maturation of Aggregates

3:26

Preparing Organoids for Cryosectioning

5:47

Immunostaining

8:27

Results: Efficient Cerebellar Differentiation and Maturation

10:09

Conclusion

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