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Bioengineering

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Nanyang Technological University

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

7.8K Views

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14:28 min

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July 15th, 2020

DOI :

10.3791/60990-v

July 15th, 2020

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Géraud Heckler¹, Christelle Aigueperse¹, Liza Hettal¹, Quentin Thuillier¹, Fabrice de Chaumont², Stéphane Dallongeville², Isabelle Behm-Ansmant¹

¹Université de Lorraine, CNRS, IMoPA F54000 Nancy France, ²Institut Pasteur, BioImage Analysis Unit, CNRS UMR 3691, Paris, France.

Summary

We present a freely available workflow built for rapid exploration and accurate analysis of cellular bodies in specific cell compartments in fluorescence microscopy images. This user-friendly workflow is designed on the open-source software Icy and also uses ImageJ functionalities. The pipeline is affordable without knowledge in image analysis.

Explore More Videos

Substructure Analyzer

Fluorescence Microscopy

Automatic Analysis

Image Segmentation

Regions Of Interest ROIs

Signal to noise Ratio

Multi channel Images

Segmentation Methods

Protocol Loading

Image Processing Workflow

Microscopy Metrics

Cellular Bodies Analysis

Chapters in this video

0:00

Introduction

1:06

Download Icy and the "Substructure Analyzer" Protocol on Icy Website

1:30

Opening the Protocol

2:03

Interacting with the Workflow on Icy

2:49

Merging of the Channels of an Image

4:57

Segmentation of the Regions of Interest

7:42

Fluorescent Signal Detection and Analysis

9:25

Run the Protocol

10:35

Representative Results

13:48

Conclusion

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