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Bioengineering

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Nanyang Technological University

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

5.3K Views

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06:53 min

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May 17th, 2020

DOI :

10.3791/60944-v

May 17th, 2020

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Yueh-Feng Wu¹, Rai-Teng Ye¹, Ming-Kai Pan²^,³, Sung-Jan Lin*¹^,³^,⁴^,⁵, Hsin-Yuan Tan*⁶^,⁷

¹Department of Biomedical Engineering, National Taiwan University, ²Institute of Pharmacology, College of Medicine, National Taiwan University, ³Molecular Imaging Center, National Taiwan University, ⁴Department of Dermatology, National Taiwan University Hospital, and College of Medicine, ⁵Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, ⁶Department of Ophthalmology, Chang Gung Memorial Hospital, ⁷College of Medicine, Chang Gung University

Summary

Presented here is a multiphoton microscopic platform for live mouse ocular surface imaging. Fluorescent transgenic mouse enables the visualization of cell nuclei, cell membranes, nerve fibers and capillaries within the ocular surface. Non-linear second harmonic generation signals derived from collagenous structures provide label-free imaging for stromal architectures.

Explore More Videos

Custom Multiphoton Microscopy

Dual Fluorescent Transgenic Mice

Titanium Sapphire Laser

Dichroic Mirrors

Band Pass Filters

Oxybuprocaine Hydrochloride

Imaging Protocol

Z Serial Imaging

Cellular Structure

Excitation Wavelengths

Chapters in this video

0:04

Introduction

0:40

Multiphoton Microscopy Setup

1:29

Eye Holder Setup

2:38

Z-Serial Image Acquisition

3:43

Image Processing and 3D Reconstruction

4:36

Results: Representative Mouse Cornea and Conjunctiva Imaging

6:10

Conclusion

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