Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
5.8K Views
•
10:01 min
•
April 8th, 2020
DOI :
April 8th, 2020
•Andreas Schreiber*1,2, Lara G. Stühn*1,2, Süreyya E. Geissinger1,2, Matthias C. Huber1,2, Stefan M. Schiller1,2,3,4,5,6
1Center for Biological Systems Analysis, University of Freiburg, 2Faculty of Biology, University of Freiburg, 3Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, 4BIOSS Centre for Biological Signalling Studies, University of Freiburg, 5IMTEK Department of Microsystems Engineering, University of Freiburg, 6Cluster of Excellence livMatS at FIT - Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg
Chapters in this video
0:05
Introduction
0:45
Protein Expression and Preparation
3:38
Dye-modification of Elastin-like Proteins via SPAAC
4:44
THF Swelling Protocol
6:29
BuOH Extrusion Protocol
7:19
Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
9:22
Conclusion
Related Videos
12:33
Origami Inspired Self-assembly of Patterned and Reconfigurable Particles
21.6K Views
07:26
Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
12.8K Views
10:22
In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions
18.1K Views
08:49
Atomically Defined Templates for Epitaxial Growth of Complex Oxide Thin Films
14.2K Views
06:35
Construction and Systematical Symmetric Studies of a Series of Supramolecular Clusters with Binary or Ternary Ammonium Triphenylacetates
8.0K Views
09:48
In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
8.2K Views
07:53
Encapsulation of Cancer Therapeutic Agent Dacarbazine Using Nanostructured Lipid Carrier
11.0K Views
09:26
Synthesis and Characterization of Supramolecular Colloids
9.7K Views
11:32
Highly Stable, Functional Hairy Nanoparticles and Biopolymers from Wood Fibers: Towards Sustainable Nanotechnology
11.9K Views
06:44
From Molecules to Materials: Engineering New Ionic Liquid Crystals Through Halogen Bonding
68.9K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved