JoVE Journal

Immunology and Infection

This content is Open Access.

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Chapters in this video

0:05

Introduction

1:03

Heat-Induced Antigen Retrieval

1:46

Nonspecific Binding Blocking

2:28

Immunofluorescence Labeling

3:36

Image Alignment

4:36

Tissue Detection

5:35

Tissue Segmentation

6:28

Cell of Interest (COI) Identification and Quantification

7:15

Tissue Heatmapping

8:10

Results: Representative Visualization, Quantification, and Mapping of Cell Populations of Interest in the Tumor Microenvironment

10:02

Conclusion

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