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Nanyang Technological UniversityA New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
4.0K Views
•
08:20 min
•
September 2nd, 2021
DOI :
September 2nd, 2021
•Chapters in this video
0:04
Introduction
1:00
Lentiviral Preparation
2:47
Determining Virus Titer and Transduction Efficacy with Flow Cytometry
4:27
Lentiviral Transduction of Target Cells and Conditional Induction of Cas9 Mediated Gene Editing
6:06
Results: Analysis of Reversibility and Rapidity of Destabilized DD-Cas9 Protein
7:24
Conclusion
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ISSN 2578-6326
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