Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
6.1K Views
•
07:26 min
•
July 29th, 2019
DOI :
July 29th, 2019
•Chapters in this video
0:04
Title
0:39
Preparation of Crude Mitochondrion from Maize Developing Kernels
1:45
RNA 5’ Polyphosphatase Treatment and Circularization
2:33
Reverse Transcription and Normalization
3:30
PCR Amplification and Determination of Transcript Termini
4:34
Verification of the cRT-PCR Mapping Results by RNA Gel Blot Hybridization
5:00
Discrimination of Primary and Processed 5’ Ends
5:26
Determination of Maize mRNA Termini Using the Circular RT-PCR-based Strategy
7:05
Conclusion
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