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Nanyang Technological University

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

9.6K Views

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08:47 min

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July 5th, 2019

DOI :

10.3791/59978-v

July 5th, 2019

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Jean-Sébastien Teoh*¹, Ming S. Soh*¹, Joseph J. Byrne*¹, Brent Neumann¹

¹Neuroscience Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne VIC 3800, Australia.

Summary

This study outlines quantitative measurements of synaptic size and localization, muscle morphology, and mitochondrial shape in C. elegans using freely available image processing tools. This approach allows future studies in C. elegans to quantitatively compare the extent of tissue and organelle structural changes as a result of genetic mutations.

Explore More Videos

Quantitative Approaches

Cellular Structures

Organelle Morphology

Caenorhabditis Elegans

Morphological Changes

Genetic Mutations

Imaging Protocols

Qualitative Methods

Fluorescence Microscopy

Chapters in this video

0:04

Title

0:59

Slide Preparation

1:48

Assessing Synaptic Morphology

2:47

Quantification of the Synaptic Region

3:45

Measurement of Muscle Cell Area

4:36

Segmentation and Quantification of Myosin Fiber Length

6:39

Results: Function of MEC-17 in the Nervous System

8:14

Conclusion

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