Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
8.8K Views
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13:04 min
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March 1st, 2019
DOI :
March 1st, 2019
•Carl Robert Rankin1, Janet Treger1, Emmanuelle Faure-Kumar2, Jihane Benhammou1, Deborah Anisman-Posner3, Alex Edward Bollinger3, Charalabos Pothoulakis1, David Miguel Padua1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles
Chapters in this video
0:04
Title
0:40
Vector Design and Generation
1:52
Virus Generation
4:24
Particle Count using p24 Enzyme-linked Immunosorbent Assay Kit
7:07
dCas9 and gRNA Transduction, Selection, and Clone Creation
10:18
Results: Using CRISPR Technology to Overexpress Multiple Splice Variants of a lncRNA
12:08
Conclusion
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