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Nanyang Technological University

An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay

10.8K Views

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09:14 min

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January 26th, 2019

DOI :

10.3791/59025-v

January 26th, 2019

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Lien Anh Ha Do¹^,², Reuben Tse¹, Jordan Nathanielsz¹, Jeremy Anderson¹, Darren Suryawijaya Ong¹, Keith Chappell³, Kim Mulholland¹^,²^,⁴, Paul V. Licciardi¹^,²

¹Murdoch Children's Research Institute, ²Department of Paediatrics, University of Melbourne, ³School of Chemistry and Molecular Bioscience, University of Queensland, ⁴London School of Hygiene and Tropical Medicine

Summary

This study describes a high throughput, imaging-based micro-neutralization assay to determine the titer of neutralizing antibodies specific for respiratory syncytial virus (RSV). This assay format has been tested on different sample types.

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Micro neutralization Assay

Neutralizing Antibodies

Respiratory Syncytial Virus

Penicillin Streptomycin

Reference Serum

Serum Test Samples

High Throughput Method

Imaging based Assay

Assay Reproducibility

Cell Seeding Protocol

Dilution Preparation

Laboratory Procedure

Chapters in this video

0:04

Title

0:48

Assay Setup

5:23

Assay Development

7:40

Results: A High-throughput Image-based RSV Neutralization Assay

8:43

Conclusion

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