Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology

34.1K Views

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09:45 min

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February 25th, 2019

DOI :

10.3791/58882-v

February 25th, 2019

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Max Z. Levine*¹^,², Nicole E. Gregorio*²^,³, Michael C. Jewett⁴^,⁵^,⁶^,⁷, Katharine R. Watts²^,³, Javin P. Oza²^,³

¹Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, ²Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, ³Department of Chemistry and Biochemistry, California Polytechnic State University, ⁴Department of Chemical and Biological Engineering, Northwestern University, ⁵Chemistry of Life Processes Institute, Northwestern University, ⁶Center for Synthetic Biology, Northwestern University, ⁷Interdisciplinary Biological Sciences Program, Northwestern University

Summary

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Escherichia Coli

Cell free Protein Synthesis

Platform Technology

Protocols

Self Re protein Synthesis

Functional Genomics

Biosensors

Metabolic Engineering

Genetic Code Expansion

Chapters in this video

0:04

Title

0:50

Cell Growth

3:30

Crude Cell Extract Preparation

5:30

Cell-Free Protein Synthesis Batch Format Reactions

6:25

Quantification of the Reporter Protein, [sfGFP]

7:24

Results: Modification and Optimization of the Cell-Free Platform Conditions

9:01

Conclusion

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