JoVE Journal

Genetics

This content is Open Access.

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

We demonstrate the use of discontinuous density gradients to separate bacterial populations based on capsule production. This method is used to compare capsule amount between cultures, isolate mutants with a specific capsule phenotype, or to identify capsule regulators. Described here is the optimization and running of the assay.

Chapters in this video

0:04

Title

0:48

Preparation of Bacterial Strains of Mutant Libraries

1:40

Preparation of Gradient Dilutions and Mini-gradient Test

2:39

Preparation of Discontinuous Density Gradients

3:39

Adding Prepared Cells to Gradients and Separation by Centrifuge

4:30

Results: Density Gradient Centrifugation Used to Separate Bacterial Strains Based on Capsule Production

5:10

Conclusion

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