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Immunology and Infection

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Nanyang Technological University

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

6.0K Views

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05:33 min

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January 7th, 2019

DOI :

10.3791/58654-v

January 7th, 2019

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Fulvia Milena Cribiù*¹, Claudia Burrello*², Roberta Tacchi¹, Francesca Boggio¹, Dario Ricca¹, Flavio Caprioli³^,⁴, Stefano Ferrero¹, Federica Facciotti²

¹Pathology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, ²Department of Experimental Oncology, European Institute of Oncology, ³Gastroenterology and Endoscopy Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, ⁴Department of Pathophysiology and Transplantation, Università degli Studi di Milano

Summary

Lack of standardization for murine tissue processing reduces the quality of murine histopathological analysis as compared to human specimens. Here, we present a protocol to perform histopathological examination of murine inflamed and uninflamed colonic tissues to show the feasibility of robotic systems routinely used for processing and embedding human samples.

Explore More Videos

Robotic Systems

Colonic Murine Samples

Histological Analyses

Automated Protocol

Histopathological Preparation

Procedural Standardization

Paraffin Embedding

Automated Processor

Embedding Technique

Processing Steps

Francesca Boggio

Chapters in this video

0:04

Title

0:47

Colon Section Preparation and Processing

2:27

Tissue Embedding

3:26

Results: Representative Murine Colon Sample Processing Analyses

4:29

Conclusion

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