A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

10.7K Views

•

09:06 min

•

October 24th, 2018

DOI :

10.3791/58617-v

October 24th, 2018

•

Jenny L. Carey¹, Olivia H. Cox¹, Fayaz Seifuddin¹, Leonard Marque¹, Kellie L.K. Tamashiro¹, Peter P. Zandi¹^,³, Gary S. Wand¹^,², Richard S. Lee¹

¹Departments of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, ²Department of Medicine, Johns Hopkins School of Medicine, ³Department of Mental Health, Johns Hopkins School of Public Health

Summary

Explore More Videos

Keywords Rat Methyl Seq

DNA Methylation

Epigenomics

Environmental Factors

Physiological Processes

Stress Exposure

Sequencing based Tool

Array based Platforms

Capture Library Hybridization Mix

Streptavidin Magnetic Beads

Chapters in this video

0:04

Title

1:04

Sample Preparation

1:54

Hybridization

4:16

Bisulfite Conversion

5:29

Indexing

6:06

Validation by Bisulfite Pyrosequencing

7:18

Results: Stress-associated DNA Methylation Changes are verified by Bisulfite Pyrosequencing

8:03

Conclusion

Related Videos

09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

10.3K Views

09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

8.4K Views

08:12

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

11.2K Views

06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

14.2K Views

00:12

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

8.4K Views

10:31

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

7.5K Views

07:10

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

4.5K Views

05:50

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

3.2K Views

11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

2.4K Views

09:10

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

3.2K Views