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Chemistry

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Nanyang Technological University

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

5.7K Views

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08:09 min

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August 6th, 2019

DOI :

10.3791/58527-v

August 6th, 2019

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Yang Wu*¹, Wei Zhang*², Jian Zhang², Zhi-Xiang Mao³, Li Ding², Hao Li³, Rong Ma¹, Jin-Hai Tang²

¹Research Center of Clinical Oncology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & Nanjing Medical University Affiliated Cancer Hospital, ²Department of General Surgery, The First Affiliated Hospital with Nanjing Medical University, ³School of Clinical Medicine, Xuzhou Medical University

Summary

This work presents the preparation of methionine functionalized biocompatible block copolymers (mBG) via the reversible addition-fragmentation chain transfer (RAFT) method. The plasmid DNA complexing ability of the obtained mBG and their transfection efficiency were also investigated. The RAFT method is very beneficial for polymerizing monomers containing special functional groups.

Explore More Videos

Block Copolymer

RAFT Polymerization

Plasmid DNA Delivery

Chapters in this video

0:04

Title

1:09

Synthesis of BNHEMA Polymer (PBNHEMA)

3:21

Synthesis of BNHEMA-b-APMA polymer (BA)

4:18

Transfection Efficiency of mBG/ GFP-pDNA Polyplexes

5:10

Results: Analysis of the Targeted Plasma DNA Delivery

7:11

Conclusion

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