Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

6.8K Views

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09:16 min

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November 26th, 2018

DOI :

10.3791/58509-v

November 26th, 2018

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Patricia Ropraz¹, Beat A. Imhof², Thomas Matthes¹, Bernhard Wehrle-Haller³, Adama Sidibé³

¹Hematology service, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, ²Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, ³Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva

Summary

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Keywords Monocyte Subpopulations

Leukocyte Adhesion

Trans endothelial Migration

Hematopoietic Cells

HUVEC

CMFDA

Density Gradient Separation

PAN Monocyte Isolation

Chapters in this video

0:04

Title

0:54

Human Umbilical Vein Endothelial Cell (HUVEC) Staining and Human Pan Monocyte Isolation

3:20

Fluidic System Preparation

5:04

Time-lapse Imaging of Monocyte Recruitment Under Flow and Image Analysis

6:56

Results: Representative Monocyte Transmigration Analysis

8:25

Conclusion

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