Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

7.6K Views

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07:22 min

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November 20th, 2018

DOI :

10.3791/58242-v

November 20th, 2018

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Lingping Wang*¹, Xinyi Wu*¹, Guojing Li¹^,², Xiaohua Wu¹, Dehui Qin³, Ye Tao⁴, Pei Xu¹^,²

¹Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, ²State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences, ³Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, ⁴Shanghai Biozeron Biotechnology Co., Ltd

Summary

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Keywords Watermelon

Bottle Gourd

Grafting

Homo And Heterografts

Cold Stress

MiRNA Response

Plant Grafting Technique

Chapters in this video

0:04

Title

1:19

Seed Sterilization and Germination

2:09

Seedling Growth and Grafting

2:48

Post-grafting Management, Cold Treatment, and Sampling

3:45

Library Preparation and High-throughput Sequencing

5:20

Results: miRNA Up- and Down-regulated in Cold Treated Homografts and Heterografts

6:17

Conclusion

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