Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

9.0K Views

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08:44 min

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August 3rd, 2018

DOI :

10.3791/58041-v

August 3rd, 2018

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Jennifer M. Brazill¹, Yi Zhu¹, Chong Li¹, R. Grace Zhai¹^,²

¹Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, ²School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University

Summary

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Keywords Quantitative Cell Biology

Neurodegeneration

Drosophila

Fluorescently Tagged Proteins

ImageJ

Image Analysis Workflow

Proteinaceous Puncta

Membrane bound Compartments

Neurodegenerative Diseases

Chapters in this video

0:04

Title

0:50

Brain Dissection and Immunofluorescence Staining

2:23

Image Acquisition and Region of Interest (ROI) Selection

4:19

Preprocessing and Segmentation with H-Maxima Watershedding and Quantity, Area, and Intensity Quantification

6:30

Results: Representative Quantitative Analysis of Cell Biology of Neurodegeneration

8:20

Conclusion

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