Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
17.3K Views
•
09:40 min
•
June 20th, 2018
DOI :
June 20th, 2018
•1Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine
Chapters in this video
0:04
Title
0:50
Preparing Collagen I Plates
1:31
Perfusion, Digestion and Harvesting Kidneys from Mice
2:36
Tissue Processing and Primary Tubular Cells Isolation
4:11
Mitochondria Bioenergetics Assay
7:26
Results: Renal Tubular Epithelial Cell (TEC) Morphology and Metabolism
9:04
Conclusion
Related Videos
09:12
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
9.0K Views
09:43
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
15.9K Views
08:06
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
18.0K Views
15:43
Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
18.1K Views
06:07
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
6.5K Views
06:41
Isolation of Primary Mouse Lung Endothelial Cells
5.0K Views
08:46
Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
9.7K Views
06:39
Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
5.5K Views
11:49
Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria
2.0K Views
04:46
Optimizing Isolation and Purification of Murine Glomerular Mesangial Cells
159 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved