A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

9.1K Views

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09:10 min

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May 22nd, 2018

DOI :

10.3791/57633-v

May 22nd, 2018

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Christoph N. Schlaffner¹^,²^,³, Georg J. Pirklbauer², Andreas Bender³, Judith A.J. Steen¹, Jyoti S. Choudhary²^,⁴

¹Department of Neurobiology, F. M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, ²Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute, Wellcome Genome Campus, ³Centre for Molecular Informatics, Department of Chemistry, University of Cambridge, ⁴Functional Proteomics Group, Chester Beatty Laboratories, Institute of Cancer Research

Summary

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Keywords Post translational Modification

Peptide Identification

Integrative Genomics Viewer

Chapters in this video

0:05

Title

0:51

Mapping of Peptides using PoGoGUI

2:36

Visualizing in the Integrative Genomics Viewer

3:57

Mapping Peptides Identified Through a Custom Variant Database to a Reference Genome

4:44

Mapping Peptides from Multiple Files Using PoGoGUI

5:48

Generating a Track Hub from Mapped Peptides and Visualizing a Track Hub in the UCSC Genome Browser

6:50

Results: Post-translational Modification and Varient Enabled Mapping of Peptides to Genomes

8:21

Conclusion

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