Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
13.4K Views
•
08:27 min
•
April 10th, 2018
DOI :
April 10th, 2018
•Lorenzo Brunetti*1,2,3, Michael C. Gundry*1,2,4, Ayumi Kitano4, Daisuke Nakada1,2,4, Margaret A. Goodell1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
Chapters in this video
0:05
Title
1:05
sgRNA Fwd Primer Design, DNA Template Synthesis, and ln Vitro Transcription of sgRNA
3:38
Cas9-sgRNA Complexing and Electroporation
5:45
Results: Use of Cas9-sgRNA Electroporation Protocol to Study Efficient Knockout of Target Gene
7:48
Conclusion
Related Videos
14:46
Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
11.0K Views
10:21
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
13.0K Views
09:04
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
8.1K Views
04:51
High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes
1.4K Views
12:04
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
3.4K Views
Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
52 Views
07:20
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
12.4K Views
08:14
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
12.1K Views
09:32
Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
552 Views
08:32
CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
3.4K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved