Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

9.1K Views

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10:34 min

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February 1st, 2018

DOI :

10.3791/57169-v

February 1st, 2018

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Christopher D. Radka¹, Lauren L. Radford², Adriana V.F. Massicano², Lawrence J. DeLucas³, Suzanne E. Lapi², Stephen G. Aller⁴

¹Graduate Biomedical Sciences Microbiology Theme, University of Alabama at Birmingham, ²Department of Radiology, University of Alabama at Birmingham, ³Office of the Provost, University of Alabama at Birmingham, ⁴Department of Pharmacology and Toxicology, University of Alabama at Birmingham

Summary

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Keywords Metal Uptake

Gram negative Bacteria

X ray Fluorescence

Radioisotopes

Cell Fractionation

ABC Transport Systems

Chapters in this video

0:04

Title

1:09

Cell Fractionation and Protein Crystallization

3:52

X-ray Fluorescence (Using GM/CA Beamline Software)

4:44

MAD Scan for Metal Energy Absorption Peak (Using GM/CA Beamline Software)

5:45

Analytical Protocol for Radioactive Metal Uptake Assay

7:24

Results: Extraction of Bacterial Periplasm from Cytoplasm in Gram-negative Bacteria

9:14

Conclusion

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