Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

9.1K Views

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14:55 min

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June 24th, 2018

DOI :

10.3791/57423-v

June 24th, 2018

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Lukasz Bozycki¹, Magdalena Komiazyk¹, Saida Mebarek²^,³^,⁴^,⁵^,⁶, Rene Buchet²^,³^,⁴^,⁵^,⁶, Slawomir Pikula¹, Agnieszka Strzelecka-Kiliszek¹

¹Laboratory of Lipid Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, ²Université de Lyon, ³Université Lyon 1, ⁴L'insitut National des Sciences Appliquées (INSA) de Lyon, ⁵Ecole Supérieure de Chimie Physique Electronique (CPE) Lyon, ⁶Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR), L'institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS)

Summary

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Keywords Mineral Nucleation

Beta glycerophosphate

Transmission Electron Microscopy

Energy Dispersive X ray Microanalysis

Chapters in this video

0:00

Title

0:38

Cell Culture and Treatment

1:26

Detection of Calcium Minerals

2:01

Visualization of Probes Under UV Light

3:28

Preparation of Probes for TEM-EDX

11:16

TEM-EDX Analysis

14:10

Conclusion

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