Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

7.0K Views

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06:51 min

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August 2nd, 2018

DOI :

10.3791/57164-v

August 2nd, 2018

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Adam C. Parslow¹^,², Andrew H.A. Clayton³, Peter Lock⁴, Andrew M. Scott¹^,²^,⁵^,⁶^,⁷

¹Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, ²School of Cancer Medicine, La Trobe University, ³Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, ⁴LIMS Bioimaging Facility, La Trobe Institute for Molecular Science, La Trobe University, ⁵Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre, Austin Health, ⁶Department of Medicine, University of Melbourne, ⁷Department of Molecular Imaging and Therapy, Austin Health

Summary

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Keywords Confocal Microscopy

Cell Surface Receptor

Aggregation

Image Correlation Spectroscopy

Chapters in this video

0:04

Title

0:39

Calculation of Image Correlation Spectroscopy

3:43

Batch Processing of ICS Datasets and Calculation of the Microscope s Beam Area

4:59

Results: Image Correlation Spectroscopy Detects Clusters on the Cell Surface of Adherent Cancer Cells

5:55

Conclusion

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