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Nanyang Technological University

Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia

12.1K Views

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06:50 min

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March 20th, 2018

DOI :

10.3791/57046-v

March 20th, 2018

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Katharina Hohlbaum¹^,², Bettina Bert²^,³, Silke Dietze², Rupert Palme⁴, Heidrun Fink², Christa Thöne-Reineke¹

¹Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine, Freie Universität Berlin, ²Institute of Pharmacology and Toxicology, Department of Veterinary Medicine, Freie Universität Berlin, ³German Federal Institute for Risk Assessment (BfR), ⁴Unit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences, University of Veterinary Medicine

Summary

We developed a protocol to assess well-being in mice during procedures using general anesthesia. A series of behavioral parameters indicating levels of well-being as well as glucocorticoid metabolites were analyzed. The protocol can serve as a general aid to estimate the degree of severity in a scientific, animal-centered manner.

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Well being Assessment

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Title

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Mouse Grimace Scale and Burrowing Behavior

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Nest Building Behavior

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Results: Assessment of Mice after General Anesthesia

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