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Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

12.6K Views

09:52 min

April 26th, 2018

DOI :

10.3791/57036-v

April 26th, 2018


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Somatic Cell Nuclear Transfer

Chapters in this video

0:05

Title

0:52

Preparation of Deionized Bovine Serum Albumin

1:48

Oocyte Collection

3:26

Preparation of Donor Cells for Nuclear Transfer and Enucleation of Oocytes

5:32

Fusion of Donor Cell and Enucleated Oocyte

7:42

Activation of Reconstructed Oocytes and Treatment with Trichostatin A and Vitamin C

8:26

Results: Somatic Cell Nuclear Transfer in Mice using Trichostatin A and Vitamin C Treatment

9:15

Conclusion

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