An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

61.1K Views

•

06:40 min

•

March 19th, 2018

DOI :

10.3791/56774-v

March 19th, 2018

•

Nastasia K-H Lim¹^,², Visse Moestrup³^,⁴, Xiao Zhang¹, Wen-An Wang⁵, Arne Møller³^,⁴^,⁶, Fu-De Huang¹^,⁴

¹Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Science, ²Shanghai Institute of Materia Medica, University of Chinese Academy of Sciences, Chinese Academy of Science, ³Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, ⁴Sino-Danish Center for Education and Research (SDC), ⁵Department of Neurology, Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiao Tong University School of Medicine, ⁶Department of Nuclear Medicine and PET-centre, Aarhus University Hospital

Summary

Explore More Videos

Keywords Cerebrospinal Fluid

CSF Collection

Anesthetized Mice

Micromanipulator

Pulled Glass Capillary

Chapters in this video

0:05

Title

0:42

Preparation for CSF Collection

1:57

Surgery to Expose the Cisterna Magna

3:34

CSF Collection

5:53

Results: Aβ ELISA Analysis of CSF Sample Collected

6:13

Conclusion

Related Videos

11:04

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

40.4K Views

09:47

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

22.9K Views

06:13

Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized Drosophila

9.8K Views

11:42

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

18.5K Views

09:44

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

14.9K Views

06:09

Piezo High Accuracy Surgical Osteal Removal (PHASOR): A Technique for Improved Cranial Window Surgery in Mice

10.6K Views

10:23

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

8.9K Views

10:04

Construction of an Improved Multi-Tetrode Hyperdrive for Large-Scale Neural Recording in Behaving Rats

11.2K Views

06:22

In Vivo Imaging of Cerebrospinal Fluid Transport through the Intact Mouse Skull using Fluorescence Macroscopy

13.6K Views

06:22

A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of Aβ and Tau Levels in Rhesus Monkey Cerebrospinal Fluid

3.6K Views