Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

10.4K Views

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09:18 min

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September 19th, 2017

DOI :

10.3791/56404-v

September 19th, 2017

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Max R Salick*¹, Michael F Wells*²^,³, Kevin Eggan²^,³, Ajamete Kaykas¹

¹Department of Neuroscience, Novartis Institutes for BioMedical Research, ²Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, ³Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University

Summary

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High throughput Assay

CRISPR

Drug Screening

Virus Pseudotyping

Chapters in this video

0:05

Title

0:43

Creating Cerebral Organoids from iPSC / hESC 2D Culture (Day 0)

3:30

Cerebral Organoid Maintenance (Day 2)

4:37

Cerebral Organoid Maintenance (Day 3)

5:14

Cerebral Organoid Maintenance (Day 4+)

6:13

Organoid Infection with ZIKV (~Day 24)

7:45

Results: Characterization of ZIKB-infected Organic Degradation

8:47

Conclusion

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