Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex

12.8K Views

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06:12 min

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January 9th, 2018

DOI :

10.3791/56263-v

January 9th, 2018

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Rong Li*¹, Jinxin Miao*¹^,², Zhiqiang Fan¹, SeokHwan Song³, Il-keun Kong³^,⁴, Yaohe Wang²^,⁵, Zhongde Wang¹

¹Department of Animal, Dairy, and Veterinary Sciences, Utah State University, ²National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, ³Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, ⁴Institute of Agriculture and Life Science, Gyeongsang National University, ⁵Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London

Summary

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Keywords CRISPR Cas9

Pronuclear Injection

Genetically Engineered

Golden Syrian Hamsters

Chapters in this video

0:05

Title

1:06

Zygote Isolation

3:31

PN Injection

4:52

Results: Evaluation of Gene Targeting in Golden Syrian Hamster by the Cas9/sgRNA System

5:36

Conclusion

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