JoVE Journal
Bioengineering
This content is Open Access.
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
18.0K Views
•
11:53 min
•
July 12th, 2017
DOI :
July 12th, 2017
•Chapters in this video
0:05
Title
0:50
Cloning of gRNAs into the CRISPER-concatemer Vector
3:40
Assessing Success of Cloning by Restriction Digest
5:28
Preparing Intestinal Organoids for Electroporation
8:08
Electroporation
10:36
Results: Successful Generation of CRISPER-concatemers and Electroporation of Intestinal Organoids
11:24
Conlcusion
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ISSN 2578-2614
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