JoVE Journal

Developmental Biology

This content is Open Access.

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

11.6K Views

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10:03 min

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August 1st, 2017

DOI :

10.3791/55914-v

August 1st, 2017

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Diana Passaro*¹, Ander Abarrategi*¹, Katie Foster¹, Linda Ariza-McNaughton¹, Dominique Bonnet¹

¹Haematopoietic Stem Cell Laboratory, The Francis Crick Institute

Summary

A method to create and live-image different humanized bone-marrow niches in mice is presented. Based on the supportive niche created by human mesenchymal cells, the addition of human endothelial cells induces the formation of human vessels, while the addition of rhBMP-2 induces the formation of human-mouse chimeric mature bone tissue.

Explore More Videos

Humanized Bone Marrow

Microenvironments

Cell Carrier Scaffolds

Haematopoietic Field

Bone Marrow Niche

Tumor Metastasis

Haematopoietic Cells

Recombinant Human Bone Morphogenetic Protein

Surgical Implantation

Chapters in this video

0:05

Title

0:54

Collagen-based Scaffold Preparation

2:40

Bioengineered Scaffold Implantation and Explant

4:43

Bioengineered Scaffold 2-Photon Microscopy Imaging

7:44

Results: Representative Scaffold Explant Analyses

9:20

Conclusion

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