Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space

11.8K Views

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10:45 min

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July 24th, 2017

DOI :

10.3791/55755-v

July 24th, 2017

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John Odackal*¹, Robert Colbourn*²^,³, Namrita Jain Odackal⁴, Lian Tao⁵, Charles Nicholson⁵, Sabina Hrabetova²

¹Department of Medicine, University of Virginia, ²Department of Cell Biology, SUNY Downstate Medical Center, ³Neural and Behavioral Science Graduate Program, SUNY Downstate Medical Center, ⁴Division of Neonatology, University of Virginia, ⁵Department of Neuroscience and Physiology, New York University School of Medicine

Summary

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Keywords Iontophoresis

Chapters in this video

0:05

Title

1:01

Real-time Iontophoresis in Agarose

3:59

Agarose Data Analysis

5:30

Real-time Iontophoresis in Brain Slices

7:02

Brain Data Analysis, Checking Transport Number and ISM Calibration

8:19

Results: Curve Fitting Data in Agar and in Brain

9:21

Conclusion

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