Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
11.2K Views
•
08:28 min
•
December 27th, 2017
DOI :
December 27th, 2017
•Elisa Peranzoni*1, Houcine Bougherara*1, Sarah Barrin*1, Audrey Mansuet-Lupo2,3, Marco Alifano4, Diane Damotte2,3, Emmanuel Donnadieu1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
Chapters in this video
0:05
Title
1:01
Agarose Embedding and Vibratome Slicing of Human Lung Tumors
3:51
Immunostaining of Resident CD8 T Cells and Tumor Cells
5:07
Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
7:03
Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
7:53
Conclusion
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