Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation

12.3K Views

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08:05 min

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July 3rd, 2017

DOI :

10.3791/55495-v

July 3rd, 2017

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Rupesh Agrawal*¹^,²^,³, Praveen Kumar Balne*², Sai Bo Bo Tun², Yeo Sia Wey², Neha Khandelwal¹, Veluchamy A. Barathi²^,⁴^,⁵

¹National Healthcare Group Eye Institute, Tan Tock Seng Hospital, ²Singapore Eye Research Institute (SERI), Singapore National Eye Center, ³School of Material Science and Engineering, Nanyang Technological University, ⁴Department of Ophthalmology, Yong Loo Lin School of Medicine, National University Health Systems, National University of Singapore, ⁵Ophthalmology Academic Clinical Research Program, DUKE-NUS Graduate Medical School

Summary

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Keywords Erythrocytes

Leukocytes

Retinal Circulation

Mouse

Fluorescent Dye Labeling

ICG

Sodium Fluorescein

Scanning Laser Ophthalmoscope

Blood Cell Flow Dynamics

Retinal Diseases

Chapters in this video

0:05

Title

0:54

Isolation and Labeling of Erythrocytes (ICG) and Leukocytes (Sodium Fluorescein)

3:25

Live-cell Imaging of Labeled Erythrocytes

5:26

Image Analysis Using MtrackJ Software

6:56

Results: Visualizaing the Labeled Erythrocytes and Leukocytes

7:38

Conclusion

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