JoVE Journal

Medicine

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Nanyang Technological University

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line

Described here is a method for producing exogenous cytokine in patient-derived xenograft (PDX) mice via weekly intraperitoneal injection of a cytokine-transduced stromal cell line. This method broadens the utility of PDX and provides the option for transient or sustained exogenous cytokine delivery in a multitude of PDX models.

Chapters in this video

0:05

Title

1:22

Culture and Expansion of Cytokine Transduced Stromal Cells

3:05

Phospho-flow Cytometry Analysis of Stroma-produced Cytokine Activity

5:09

Injection of Cytokine-transduced Stroma into Mice

6:22

Serial Blood Collection and Plasma Monitoring of Exogenous Human Cytokine in Mice

8:33

Human Hematopoietic Cell Transplantation

9:53

Results: Achieving Physiological Levels of the Human TSLP Cytokine in PDX Mice

11:22

Conclusion

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