Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells

4.1K Views

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12:02 min

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November 11th, 2021

DOI :

10.3791/60170-v

November 11th, 2021

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Shuhan Meng¹^,², Aaron G. Whitt¹^,²^,³, Allison Tu², John W. Eaton¹^,²^,³, Chi Li¹^,²^,³, Kavitha Yaddanapudi⁴^,⁵^,⁶

¹Department of Pharmacology and Toxicology, University of Louisville, ²Experimental Therapeutics Program, Brown Cancer Center, University of Louisville, ³Department of Medicine, University of Louisville, ⁴Department of Surgery, University of Louisville, ⁵Immuno-Oncology Program, Brown Cancer Center, University of Louisville, ⁶Department of Microbiology and Immunology, University of Louisville

Summary

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Exosome

Extracellular Vesicles

Immune Regulatory Vesicles

Molecular Biology Techniques

Chapters in this video

0:04

Introduction

1:01

ES-D3 Cell Culture

3:06

Exosome-Enriched Extracellular Vesicle (EV) Isolation

4:09

Exosome-Enriched EV Transmission Electron Microscopy (TEM) Characterization

5:12

Whole Cell Extract (WCE) and EV Lysate Preparation

6:29

Western Blot Analysis

7:11

Enzyme-Linked Immunosorbent Assay (ELISA)

8:48

Results: Representative GM-CSF Expression Analyses

11:13

Conclusion

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