Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
19.1K Views
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07:04 min
•
February 11th, 2017
DOI :
February 11th, 2017
•Sylvia F. Boj1, Annelotte M. Vonk2, Marvin Statia1, Jinyi Su1, Johanna F. Dekkers3, Robert R. G. Vries1, Jeffrey M. Beekman2, Hans Clevers1,4
1Foundation Hubrecht Organoid Technology, 2Department of Pediatric Pulmonology, Regenerative Medicine Centre Utrecht, Wilhelmina Children's Hospital, University Medical Centre Utrecht, 3Department of Stem Cells and Cancer, Walter and Eliza Hall Institute of Medical Research, 4Hubrecht Institute for Developmental Biology and Stem Cell Research, University Medical Centre Utrecht
Chapters in this video
0:05
Title
1:06
Colon Organoid Passaging
3:56
Forskolin-induced Swelling Assay (FIS)
5:29
Results: Representative FIS Quantification
6:14
Conclusion
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