Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction

7.7K Views

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17:05 min

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July 8th, 2017

DOI :

10.3791/55122-v

July 8th, 2017

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Dmitry V. Samigullin¹^,²^,³, Eduard F. Khaziev¹^,²^,³, Nikita V. Zhilyakov¹^,², Ellya A. Bukharaeva¹^,², Eugeny E. Nikolsky¹^,²^,⁴

¹Laboratory of Biophysics of Synaptic Processes, Kazan Scientific Centre, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, ²Open Laboratory of Neuropharmacology, Kazan Federal University, ³Department of Radiophotonics and Microwave Technologies, A.N. Tupolev Kazan National Research Technical University, ⁴Department of Medical and Biological Physics, Kazan State Medical University

Summary

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Keywords Calcium Dye

Frog Nerve Endings

Neuromuscular Junction

Chapters in this video

0:00

Title

0:06

Introduction

1:03

Preparing of a Dye Loading Solution

1:53

Dye Loading Procedure

5:40

Praraing the Tissue for Microscopy

8:22

Video Capture with Digital CCD Camera

10:06

Data Analysis

14:13

Results

14:56

Discussion

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