Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

11.2K Views

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06:29 min

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December 22nd, 2016

DOI :

10.3791/54956-v

December 22nd, 2016

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Chi Ching Goh¹^,², Jackson LiangYao Li¹, David Becker³, Wolfgang Weninger⁴^,⁵^,⁶, Veronique Angeli²^,⁷, Lai Guan Ng¹^,²^,⁵^,⁸

¹Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, ²Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, ³Lee Kong Chian School of Medicine, Nanyang Technological University, ⁴Centenary Institute for Cancer Medicine and Cell Biology, ⁵Discipline of Dermatology, University of Sydney, ⁶Department of Dermatology, Royal Prince Alfred Hospital, ⁷LSI Immunology Programme, National University of Singapore, ⁸School of Biological Sciences, Nanyang Technological University

Summary

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Keywords Ischemia reperfusion Injury

Mouse Ear Skin

Intravital Multiphoton Imaging

Immune Response

Skin Inflammation

Hypersensitivity Reactions

Chapters in this video

0:05

Title

0:53

Mouse Anesthesia and Depilation

1:35

Induction of Ischemia and Reperfusion Injury

2:31

Placement of the Ear on the Imaging Platform

4:33

Results: Neutrophil Recruitment in Ischemia-reperfusion Injury

5:20

Conclusion

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