Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
9.9K Views
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10:30 min
•
December 8th, 2016
DOI :
December 8th, 2016
•Luisa Trombi1,2, Serena Danti2,3, Sara Savelli4, Stefania Moscato1, Delfo D'Alessandro2,5, Claudio Ricci2, Stefano Giannotti6, Mario Petrini1
1Dept. of Clinical and Experimental Medicine, University of Pisa, 2OtoLab, Azienda Ospedaliero-Universitaria Pisana (AOUP), 3Dept. of Civil and Industrial Engineering, University of Pisa, 4Immunohematology Operative Unit, Azienda Ospedaliero-Universitaria Pisana (AOUP), 5Dept. Of Surgical, Medical, Molecular Pathology and Emergency Medicine, University of Pisa, 6II Orthopedic and Traumatologic Clinic, Azienda Ospedaliero-Universitaria Pisana (AOUP)
Chapters in this video
0:05
Title
0:57
Preparation of Autologous Serum
2:20
Isolation of hMSCs from Bone Marrow
3:42
Culture of hMSCs in the Presence of Autologous Serum
5:19
Osteogenic Pre-induction and Recovery of hMSCs
6:20
Preparation of hMSCs/Fibrin Clot Constructs and Cell Viability Assay
7:30
Results: hMSCs/Fibrin Clot Generation and Application
9:10
Conclusion
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