Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
8.2K Views
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12:19 min
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November 10th, 2016
DOI :
November 10th, 2016
•Chapters in this video
0:05
Title
0:50
Transfection of TFO-directed ICL-containing Plasmids in Human Cells
2:27
Crosslinking and DNA Isolation
5:21
Chromatin Immunoprecipitation of TFO-directed ICL-containing Plasmids
7:06
DNA Supercoiling Assay and Two Dimensional Agarose Gel Electrophoresis
9:30
Studying DNA Topology Modification Using ChIP and DNA Supercoiling Assays
10:45
Conclusion
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