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Nanyang Technological University

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

11.5K Views

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08:09 min

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August 4th, 2016

DOI :

10.3791/54401-v

August 4th, 2016

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Huazheng Liang*¹^,², Bassem Akladios*¹, Cesar P. Canales*¹, Richard Francis³, Edna H. Hardeman¹, Annemiek Beverdam¹

¹The School of Medical Sciences, University of New South Wales Australia, ²Neuroscience Research Australia, ³Biomedical Imaging Facility, University of New South Wales Australia

Summary

This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.

Explore More Videos

Protein Expression

Single Cell Resolution

Whole Mount Skin Preparations

Epidermal Development

Skin Regeneration

CUBIC Clearing Solution

Chapters in this video

0:05

Title

1:19

Transparent Mouse Skin Tissue Preparation

2:32

Mouse Skin Biopsy Clearance

4:27

Mouse Skin Biopsy Imaging

5:08

Results: Single Cell Resolution Protein Expression in Whole Mount Skin Preparations

6:55

Conclusion

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