A Robotic Platform for High-throughput Protoplast Isolation and Transformation

14.2K Views

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10:12 min

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September 27th, 2016

DOI :

10.3791/54300-v

September 27th, 2016

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Elizabeth M. Dlugosz¹, Scott C. Lenaghan²^,³, C. Neal Stewart, Jr.¹

¹Department of Plant Sciences, University of Tennessee, Knoxville, ²Center for Renewable Carbon, University of Tennessee, Knoxville, ³Department of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, Knoxville

Summary

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Keywords Protoplast Isolation

High throughput Screening

Plant Biotechnology

Automated Protoplast Production

Protoplast Transformation

BY 2 Suspension Culture

Microplate Mover

Liquid Handler

Multi mode Plate Reader

Start And End Positions

Chapters in this video

0:05

Title

0:40

Establishment of Suspension Cell Cultures

1:40

Set up of Automated Protocols for Protoplast Isolation and Transformation

5:16

Automated Protocols

6:37

Microscopic Analysis of Transformation

7:42

Results: Rapid, Automated, High-throughput Screening of Plant Protoplasts

9:40

Conclusion

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