A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

16.4K Views

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06:28 min

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August 12th, 2016

DOI :

10.3791/54255-v

August 12th, 2016

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Clovis S. Palmer¹^,²^,³, Joshua J. Anzinger⁴, Tiffany R. Butterfield⁴, Joseph M. McCune⁵, Suzanne M. Crowe¹^,²^,⁶

¹Centre for Biomedical Research, Macfarlane Burnet Institute for Medical Research and Public Health, ²Department of Infectious Diseases, Monash University, ³Department of Microbiology and Immunology, University of Melbourne, ⁴Department of Microbiology, The University of the West Indies, ⁵Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, ⁶Department of Medicine, Monash University

Summary

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Keywords Flow Cytometry

Glucose Uptake

Glucose Transporter

Monocyte Subpopulations

Fluorescent Glucose Analog

FACS

Chapters in this video

0:05

Title

0:43

Monocyte and Monocyte Subpopulation Glucose Transporter 1 (Glut1) Cell Surface Detection

2:07

Monocyte Glucose Uptake Analysis

3:11

Data Acquisition and Analysis

4:16

Results: Representative Monocyte Subpopulation Glucose Uptake Analysis

5:22

Conclusion

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